哪里有样本释放剂操作方法
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哪里有样本释放剂操作方法
广州健仑生物科技有限公司
（广州健仑生物科技有限公司是集研制开发、销售、服务于一体的优良企业,公司产品涉及临床快速诊断试剂、食品安全检测试剂，违禁品快速检测，动物疾病防疫检测试剂，免疫诊断试剂、临床血液学和体液学检验试剂、微生物检验试剂、分子生物学检验试剂、临床生化试剂、有机试剂等众多领域，同时核心代理Panbio、FOCUS、Qiagen、IBL、CORTEZ、Fuller、Inbios、BinaxNOW、LumuQuick、日本富士、日本生研等多家有名诊断产品集团公司产品，致力于为商检单位、疾病预防控制中心、海关出入境检疫局、卫生防疫单位,缉毒系统，戒毒中心，检验检疫单位、生化企业、科研院所、医疗机构等机构与行业提供*、高品质的产品服务。此外，本公司还开展食品、卫生、环境、药品等多方面的第三方检测服务。）
哪里有样本释放剂操作方法
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【产品介绍】
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【公司名称】 广州健仑生物科技有限公司
【】 杨永汉
【】
【腾讯 】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-3室
【企业文化】
【Product Name】 Sample Release Agent
【Packing】 20 test / box (solution I: 20 × 1 Test / bottle; solution II: 20 Test / bottle)
50 test / box (solution I: 50x1 Test / bottle; solution II: 50 Test / bottle X1)
100 test / box (solution I: 100 x 1 Test / bottle; solution II: 50 Test / bottle x 2)
Detection method
(A) discharge antibody detection
Test tube method
(1) Label the test tube and add 1 drop of erythrocyte suspension of 2% known blood type, add 2 drops of the bleaching solution, mix and incubate at 37 ℃ for 30 minutes.
(2) Remove the test tube and wash the cells 3 times with saline. After the last wash, thoroughly decant the washing solution and blot the water in the test tube with absorbent paper.
(3) Add 2 drops of anti-human globulin reagent and mix well and centrifuge at 1000g for 15 seconds.
(4) gently shake the test tube, naked eye or under the microscope to read the results of agglutination.
2. Micro-column gel method
(1) Take anti-human globulin test card and make the mark, add 50μL of 0.8 ~ 1% red blood cell suspension of known blood type to the hole.
(2) Add 50 μL of the dispersion to the wells and incubate at 37 ° C for 15 minutes. Centrifuge using a card-type centrifuge.
(3) Remove the microcolumn gel card, the naked eye or interpreter read the agglutination results.
(B) detection of red cell antigen released after dissociation
1. To IgM monoclonal antibody detection of red blood cell antigen
(1) The dissociated red blood cells were washed 6 times with physiological saline, and then prepared into about 2% erythrocyte suspension with normal saline.
(2) Add 2 drops of antibody to a clean tube and label.
(3) Add 1 drop of 2% of the above suspension of erythrocytes to the test tube, gently mix and centrifuge at 1000g for 15 seconds.
(4) gently shake the test tube, naked eye or under the microscope to read the results of agglutination.
2. IgG monoclonal antibody detection of red blood cell antigen
(1) The dissociated red blood cells were washed 6 times with physiological saline, and then prepared into about 2% erythrocyte suspension with normal saline.
(2) Add 2 drops of antibody to a clean tube and label.
(3) Add 1 drop of 2% of the suspension of erythrocytes to be tested to the test tube and mix well and incubate in a water bath at 37 ° C. for 30 minutes.
(4) Remove the test tube and wash the cells three times with saline. After the last wash, thoroughly decant the washing solution and blot the water in the test tube with absorbent paper.
(5) Add 2 drops of anti-human globulin reagent and mix well and centrifuge at 1000g for 15 seconds.
(6) Gently shake the tube and read the agglutination results either naked or under the microscope.
【Reference】 This product is qualitative reagent, do not need to set the reference value.
[Interpretation of test results]
1. Positive reaction: the discharge and the corresponding erythrocytes in the test tube or agglutination of the corresponding agglutination of the distribution of the red blood cells in the gel surface or gel, indicating that the red blood cells have been sensitized by the antibody, the antibody solution.
2. Negative reactions: the discharge solution and the corresponding red blood cells in the test tube does not appear agglutination or the corresponding deposition of all red blood cells in the bottom of the gel tube, indicating that the red blood cells were not sensitized by the antibody, the discharge solution does not contain antibodies.
Limitations of test methods
1. Diluent can not be used to identify Duffy blood group antibody.
2. The erythrocytes treated by this kit can not be used for the phenotype detection of Kell's blood group system.
【Product Performance Index】
1. pH value (25 ° C ± 1 ° C): 1.7 ± 0.2 (Solution I) 9.0 ± 0.2 (Solution II)
2. Osmolarity: 450 ± 20 mOsm / L (Solution I) 130 ± 20 mOsm / L (Solution II)
【Precautions】
1. Diluent and the last wash cell supernatant to do parallel testing to confirm the serum free antibody has been compley removed.
2. Dissolution liquid test tube method, if the negative reaction, you need to add human IgG anti-D sensitized erythrocytes to confirm the test to ensure the effectiveness of anti-human globulin reagents.
3. Dissolution liquid added to the solution II to adjust the pH to neutral prone to sediment turbidity can be centrifuged to remove the precipitate after supernatant antibody detection.
