西尼罗河病毒检测试剂盒（PCR-荧光探针法）

西尼罗河病毒检测试剂盒（PCR-荧光探针法）

广州健仑生物科技有限公司

One tube multiplex for detection of West Nile virus and internal control

单管多重检测西尼罗河病毒和内部对照

西尼罗河病毒检测试剂盒（PCR-荧光探针法）

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

一、基本知识与原理 
转导是以噬菌体为媒介将一个细胞的遗传物质转移给另一个细胞的过程。随着分子遗传学的发展，转身已成为基因精细结构分析的常用方法之一。 
根据噬菌体转导供体菌基因的差异，转导可分为普遍性转导和局限性转导。这里以局限性转导为例说明转导的基本原理。局限性转导实验中常用的是大噬菌体，它能整合在大肠杆菌染色体DNA上的半乳糖基因（gAl）和生物素基因（bib）之间，因此，它能转导半乳糖基因（一）又能转导生物素基因（bio）。 
本实验选用大肠杆菌 E。oh K．2（A）为供体菌（即大噬菌体的DNA已整合在大肠杆菌的DNA上，我们称该大肠杆菌为溶源性大肠杆菌，’gAT””为带有半乳糖基因）。由于在此供体菌中噬菌体与半乳糖基因（匆”）紧密连锁，因此，当此供体菌受紫外线照射后会产生裂解反应，噬菌体被诱发释放，以一定的比例形成带有半乳糖基因的转导噬菌体。当这种转导噬菌体与受体菌 ECo］i K；。 s gAl－（此细菌不能利用半乳糖，‘’表示此细菌半乳糖基因发生突变）混合接触时，带有一基因的转导噬菌体能以一定的频率整合到受体首上，从而使不能利用半乳糖的 gAl一受体菌转变成了能利用半乳糖的 gAT”细菌。整个过程可用图12－’表示：l 
二、实验目的和要求 
以局限性转导为例来说明转导的基本原理，进一步验证是遗传物质，并初步掌握转导实验的基本方法。 
三、实验器材 
（1）实验材料：供体菌 受体菌 
（2）实验试剂：肉汤液体培养基、肉汤固体培养基、ZE 肉汤液体培养基、半固体琼脂培养基、半乳糖Emb培养基、灭菌生理盐水（或磷酸缓冲液） 
D（3）实验设备：培养皿（9厘米）、三角烧瓶（50毫升）、试管、离心管，离心机，移液管，涂棒，水浴锅，离心机，紫外照射箱、温箱 

First, the basic knowledge and principles
Transduction is the process of transferring a cell's genetic material to another cell using phage as a medium. With the development of molecular genetics, turning has become one of the commonly used methods for gene fine structure analysis.
According to the phage transduction donor gene differences, transduction can be divided into universal transduction and localized transduction. Here is a case of limited transduction as an example of the basic principle of transduction. Commonly used in limited transduction experiments is the large phage, which integrates between the galactose gene (gAl) and the biotin gene (bib) on the chromosomal DNA of E. coli so that it can transduce the galactose gene (a) But also biotin gene (bio).
E. coli E used in this experiment. oh K. 2 (A) is the donor bacteria (ie, the DNA of the phage is integrated into the DNA of E. coli, which we call lysogenic E. coli and the "gAT" is the galactose-bearing gene). Since the bacteriophages are closely linked to the galactose gene (hASH) in this donor bacterium, when the donor bacterium is lysed by UV rays, a cleavage reaction occurs and the phage are induced to release, forming a certain ratio of the galactose gene Of the transduced phage. When this transduced phage and recipient bacteria ECo] i K ;. s gAl- (the bacteria can not use galactose, '' means that the bacterial galactose gene mutation) mixed contact with a gene Of the transductant phage can be integrated into the receptor at a certain frequency on the first, so that can not use galactose gAl a receptor bacteria into galactose gAT "bacteria. The whole process can be shown in Figure 12- ': l
Second, the purpose and requirements of the experiment
The limitations of transduction as an example to illustrate the basic principles of transduction, further validation of genetic material, and preliminary grasp of the basic methods of transduction experiments.
Third, experimental equipment
(1) Experimental material: donor bacteria bacteria
(2) Experimental Reagents: broth broth, broth solid broth, ZE broth broth, semi-solid agar broth, galactose Emb medium, sterile saline (or phosphate buffer)
(3) Experimental equipment: Petri dishes (9 cm), Erlenmeyer flask (50 ml), test tube, centrifuge tube, centrifuge, pipette, dip stick, water bath, centrifuge, UV irradiation box,