细菌性脑膜炎3重核酸检测试剂盒（PCR方法）

细菌性脑膜炎3重核酸检测试剂盒(PCR方法)

广州健仑生物科技有限公司

准备使用lyo master混合物（8孔条）检测脑膜炎奈瑟菌（Nmeng），肺炎链球菌（Spneu）和流感嗜血杆菌（Haeinf），包括内部对照。
Ready to use lyo master mix (8-well strips) for detection of Neisseria meningitidis (Nmeng), Streptococcus pneumoniae (Spneu) and Haemophilus influenzae (Haeinf) including an internal control.

细菌性脑膜炎3重核酸检测试剂盒(PCR方法)

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细菌性脑膜炎3重核酸检测试剂盒(PCR方法)

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

细菌、酵母菌，放线菌和霉菌；
肉膏蛋白胨斜面培养基，灭菌脱脂牛乳，灭菌水，化学纯的液体石蜡，甘油，五氧化二磷，河沙，瘦黄土或红土，冰块、食盐，干冰，95％酒精，10％盐酸，无水氯化钙；
灭菌吸管，灭菌滴管，灭菌培养皿，管形安瓿管，泪滴形安瓿管（长颈球形底），40目与 100目筛子，油纸，滤纸条（0．5×1．2cm），干燥器，真空泵，真空压力表，喷灯，L形五通管，冰箱，低温冰箱（-30℃），液氮冷冻保藏器。
四、操作步骤、各保藏法的应用范围及优缺点
下列各法可根据实验室具体条件与需要选做。
1．斜面低温保藏法
将菌种接种在适宜的固体斜面培养基上，待菌充分生长后，棉塞部分用油纸包扎好，移至2—8℃的冰箱中保藏。
保藏时间依微生物的种类而有不同，霉菌、放线菌及有芽孢的细菌保存2—4个月，移种一次。酵母菌两个月，细菌每月移种一次。
此法为实验室和工厂菌种室常用的保藏法，优点是操作简单，使用方便，不需特殊设备，能随时检查所保藏的菌株是否死亡、变异与污染杂菌等。缺点是容易变异，因为培养基的物理、化学特性不是严格恒定的，屡次传代会使微生物的代谢改变，而影响微生物的性状；污染杂菌的机会亦较多。
2．液体石蜡保藏法
（1）将液体石蜡分装于三角烧瓶内，塞上棉塞，并用牛皮纸包扎，1．05kg/cm2>，121．3℃灭菌30分钟，然后放在40℃温箱中，使水汽蒸发掉，备用。
（2）将需要保藏的菌种，在zui适宜的斜面培养基中培养，使得到健壮的菌体或孢子。
（3）用灭菌吸管吸取灭菌的液体石蜡，注入已长好菌的斜面上，其用量以高出斜面顶端1cm为准（图Ⅶ-12），使菌种与空气隔绝。
Third, equipment
Bacteria, yeasts, actinomycetes and molds;
Cream peptone slant medium, sterilized skim milk, sterilized water, chemically pure liquid paraffin, glycerol, phosphorus pentoxide, river sand, thin yellow or red clay, ice, salt, dry ice, 95% Hydrochloric acid, anhydrous calcium chloride
Sterilizing pipette, sterilizing pipette, sterilizing petri dish, ampule tube ampule, teardrop ampule tube (long neck spherical bottom), 40 mesh and 100 mesh sieve, paper, filter paper (0.5 × 1. 2cm), dryer, vacuum pump, vacuum gauge, torch, L-shaped five-tube, refrigerator, low temperature refrigerator (-30 ℃), liquid nitrogen freezer.
Fourth, the operational steps, the scope and advantages and disadvantages of each deposit law
The following methods can be selected according to the specific conditions and needs of the laboratory.
1. Inclined surface temperature preservation method
The strain was inoculated on a suitable solid slant culture medium, to be fully grown bacteria, cotton tampon partially wrapped with oil paper, moved to 2-8 ℃ refrigerator preservation.
Storage time varies according to the type of microorganisms, molds, actinomycetes and spores of bacteria stored for 2-4 months, transplanted once. Yeast two months, the bacteria is best to move once a month.
This method is commonly used in laboratories and factory bacteria room preservation method, the advantages of simple operation, easy to use, without special equipment, can check the deposited strains at any time whether the death, mutation and contamination of bacteria. The disadvantage is that it is easy to mutate because the physical and chemical properties of the medium are not strictly constant. Repeated passages will change the metabolism of the microorganisms and affect the characteristics of the microorganisms. There is also more chance of contamination of the bacteria.
2. Liquid paraffin deposit method
(1) Dispense the liquid paraffin into a conical flask, tampon it, and wrap it with kraft paper 1.05kg / cm2> and sterilize it at 121.3 ° C for 30 minutes and then place it in a 40 ° C incubator to evaporate the water vapor Off, spare.
(2) The strain to be preserved, cultured in the most appropriate slant medium, so that robust bacteria or spores.
(3) The sterilized liquid paraffin is drawn in with a sterile pipette and injected into the slant surface which has grown well. The dosage is about 1cm above the top of the bevel (Figure VII-12), isolating the strain from the air.
(4) Keep the tube upright, store at low temperature or at room temperature (some microorganisms are longer than the refrigerator at room temperature).