- 产品描述
卡氏肺孢子虫检测试剂盒(PCR-荧光探针法)
广州健仑生物科技有限公司
One tube multiplex for detection AND quantification of Pneumocystis jirovecii and internal control.
一个管多重检测和定量的卡氏肺孢子虫和内部控制。
Principle
Multiplex real-time PCR for detection of pathogen genes by TaqMan® technology
Targets
One tube multiplex PCR:
Pneumocystis jirovecii
internal control
Specimen
This test is for use with extracted nucleic acid from respiratory samples (throat/nasal swabs, bronchoalveolar lavage and sputum) of human origin
Storage
-20°C
Shelf life
12 months from manufacture
卡氏肺孢子虫检测试剂盒(PCR-荧光探针法)
货号 | 产品名称 | 英文名称 |
JL-FT001 | 呼吸道病原体21种多重检试剂盒(PCR方法) | Respiratory pathogens 21 |
JL-FT002 | 21种呼吸道病原体联合检试剂盒(PCR方法) | Respiratory pathogens 21 |
JL-FT003 | 呼吸道病原体25联检测试剂盒(PCR方法) | Respiratory pathogens 25 plus |
JL-FT004 | 33种呼吸道病原体联合检测试剂盒(PCR方法) | Respiratory pathogens 33 |
JL-FT005 | 8种细菌性肺炎多重检测试剂盒(PCR方法) | Bacterial pneumoniae CAP |
JL-FT006 | 4种非典型肺炎联合检测试剂盒(PCR方法) | Atypical CAP |
JL-FT007 | 肺炎克雷伯菌/铜绿假单胞菌联合检测试剂盒(PCR方法) | Bacterial pneumoniae HAP |
JL-FT008 | 博德特氏菌检测试剂盒(PCR-荧光探针法) | Bordela |
JL-FT009 | 3种流感病毒检测试剂盒(PCR-荧光探针法) | FLU |
JL-FT010 | 中东呼吸综合征冠状病毒(MERS-CoV)检测试剂盒(PCR方法) | MERS-CoV |
JL-FT011 | MERS-CoV 中东呼吸综合征冠状病毒PCR检测试剂盒 | MERS-CoV |
JL-FT012 | Pneumocystis jirovecii | |
JL-FT013 | 流感甲乙型/人呼吸道合胞病毒AB型四联检测试剂盒(PCR-荧光探针法) | FLU/HRSV |
JL-FT014 | 人呼吸道合胞病毒AB型和流感病毒甲乙型联合检测PCR试剂盒 | FLU/HRSV |
JL-FT015 | 军团菌属三通道多重检测试剂盒(PCR-荧光探针法) | Legionella |
JL-FT016 | 人冠状病毒NL63、 229E、OC43 and HKU1联合检测试剂盒(PCR方法) | HCoV |
我司还提供其它进口或国产试剂盒:登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。
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【公司名称】 广州健仑生物科技有限公司
【市场部】 杨永汉
【】
【腾讯 】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室
三、第二抗体的染色
12、加100 ul(2滴)双染增强剂(试剂7),孵育;
13、用TPBS冲洗3 min×3次;
14、加100 ul(2滴)血清封闭溶液(试剂1)到切片上,室温孵育10 min,倒掉(不能冲洗);
15、加入稀释好的一抗(按照说明推荐浓度和孵育条件);
16、用TPBS冲洗5 min×3次;
17、加入100 ul(2滴)生物素化的二抗(试剂2),室温孵育10 min;
18、用TPBS冲洗5 min×3次;
19、加入100 ul(2滴)链霉亲和素-HRP(试剂5)的二抗,室温孵育10 min;
20、用TPBS冲洗5 min×3次;
21、 准备底物溶液:将1滴试剂6A和6B加入到1 ml去离子水中,充分混匀。再加入1滴试剂6C,充分混匀;
22、 加100 ul(2滴)配好的HRP底物溶液,室温孵育(镜下掌握孵育时间);
23、 使用含有0.05%吐温20的去离子水冲洗终止反应;
24、 使用复染试剂染色(根据需要选择);
25、 封片剂(试剂8)封片,显微镜观察并拍照。
Third, the second antibody staining
12, add 100 ul (2 drops) double staining enhancer (reagent 7), incubation;
13, rinsed with TPBS 3 min × 3 times;
14. Add 100 ul (2 drops) serum blocking solution (Reagent 1) to the sections and incubate at room temperature for 10 min.
15, add a good dilution of the first antibody (according to the instructions recommended concentration and incubation conditions);
16, rinsed with TPBS 5 min × 3 times;
17. Add 100 ul (2 drops) biotinylated secondary antibody (Reagent 2) and incubate for 10 min at room temperature.
18, rinsed with TPBS 5 min × 3 times;
19. Add 100 ul (2 drops) streptavidin-HRP (Reagent 5) secondary antibody and incubate for 10 min at room temperature.
20, rinsed with TPBS 5 min × 3 times;
21. Prepare Substrate Solution: Add 1 drop of Reagents 6A and 6B to 1 ml of deionized water and mix well. Then add 1 drop of reagent 6C, mix well;
22, add 100 ul (2 drops) with a good solution of HRP substrate, incubated at room temperature (microscopy grasp the incubation time);
23. Stop the reaction by rinsing with deionized water containing 0.05% Tween 20;
24, using stained reagents staining (according to need to choose);
25, sealing agent (reagent 8) sealing, microscopic observation and photographing.