- 产品描述
博德特氏菌检测试剂盒(PCR-荧光探针法)
广州健仑生物科技有限公司
One tube multiplex for detection AND quantification of Bordela spp. and internal control.
单管多重检测和定量博德特氏菌属和内控。
博德特氏菌检测试剂盒(PCR-荧光探针法)
货号 | 产品名称 | 英文名称 |
JL-FT001 | 呼吸道病原体21种多重检试剂盒(PCR方法) | Respiratory pathogens 21 |
JL-FT002 | 21种呼吸道病原体联合检试剂盒(PCR方法) | Respiratory pathogens 21 |
JL-FT003 | 呼吸道病原体25联检测试剂盒(PCR方法) | Respiratory pathogens 25 plus |
JL-FT004 | 33种呼吸道病原体联合检测试剂盒(PCR方法) | Respiratory pathogens 33 |
JL-FT005 | 8种细菌性肺炎多重检测试剂盒(PCR方法) | Bacterial pneumoniae CAP |
JL-FT006 | 4种非典型肺炎联合检测试剂盒(PCR方法) | Atypical CAP |
JL-FT007 | 肺炎克雷伯菌/铜绿假单胞菌联合检测试剂盒(PCR方法) | Bacterial pneumoniae HAP |
JL-FT008 | Bordela | |
JL-FT009 | 3种流感病毒检测试剂盒(PCR-荧光探针法) | FLU |
JL-FT010 | 中东呼吸综合征冠状病毒(MERS-CoV)检测试剂盒(PCR方法) | MERS-CoV |
JL-FT011 | MERS-CoV 中东呼吸综合征冠状病毒PCR检测试剂盒 | MERS-CoV |
JL-FT012 | 卡氏肺孢子虫检测试剂盒(PCR-荧光探针法) | Pneumocystis jirovecii |
JL-FT013 | 流感甲乙型/人呼吸道合胞病毒AB型四联检测试剂盒(PCR-荧光探针法) | FLU/HRSV |
JL-FT014 | 人呼吸道合胞病毒AB型和流感病毒甲乙型联合检测PCR试剂盒 | FLU/HRSV |
JL-FT015 | 军团菌属三通道多重检测试剂盒(PCR-荧光探针法) | Legionella |
JL-FT016 | 人冠状病毒NL63、 229E、OC43 and HKU1联合检测试剂盒(PCR方法) | HCoV |
我司还提供其它进口或国产试剂盒:登革热、疟疾、流感、A链球菌、合胞病毒、腮病毒、乙脑、寨卡、黄热病、基孔肯雅热、克锥虫病、违禁品滥用、肺炎球菌、军团菌、化妆品检测、食品安全检测等试剂盒以及日本生研细菌分型诊断血清、德国SiFin诊断血清、丹麦SSI诊断血清等产品。
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【公司名称】 广州健仑生物科技有限公司
【市场部】 杨永汉
【】
【腾讯 】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室
4、捞组织
当组织载玻片置于40 oC温水中之前,要先将水浴中的气泡赶走,以免气泡受热上浮而贴到组织上,组织受热展开,是组织不起皱纹,用载玻片捞组织时,一般取载玻片的下1/3或者下1/2一般每种组织捞5-6张,其中2-3张是备用的,每张载玻片上通常捞两份组织,做对照使用,这样形成的误差就比较小了,而且捞载玻片的时候方向*,以便观察,再将捞出来的载玻片置于架子上,放入37 oC温箱中烘干。
5、脱蜡
依次将载玻片放入二甲苯-二甲苯-100%酒精-100%酒精-95%酒精-90%酒精-80%酒精-70%酒精,起脱蜡作用的主要是二甲苯,依据的是相似相溶的原理.一般在每个试剂中放10 min,天气热可以少放几分钟,相反,天气较冷的话就要适当延长脱蜡时间,一般为12-15 min.
6、抗原修复
脱蜡后在清水中冲洗一段时间,加入3%H2O2浸泡10 min,从而除去内源性的过氧化氢酶,然后倒掉H2O2,在清水中洗两次,再加入柠檬酸缓冲液,放入微波炉中蒸煮3 min(中火),一般刚到沸腾即可,冷却至室温,然后再蒸煮一次,冷却至室温,蒸煮的目的是为了使抗原的位点暴露出来。
7、血清封闭
冷却至室温后,将柠檬酸缓冲液倒掉,水洗2次,并将载玻片置于PBS中5 min,洗2次,擦干组织周围的PBS液,马上加上血清,使一些非特异性的位点封闭起来,然后放入37 oC温箱中半小时。血清稀释10倍(900 ul PBS:100 ul血清封闭液)。
4, fishing organizations
When the tissue slides placed in 40 oC warm water, the first bubble in the water bath away, so as not to heat the bubbles floating and affixed to the tissue, heat tissue, it is best not to organize wrinkles, slide Tissue, the general take slides under 1/3 or 1/2 generally fishing 5-6 each tissue, of which 2-3 are spare, each slide is usually fishing two organizations, as a control Use, so the error is relatively small, but when fishing slides best in the same direction, in order to observe, and then remove the slide placed on the shelf, placed in a 37 oC incubator drying.
5, dewaxing
Slides were loaded sequentially into xylene-xylene-100% alcohol-100% alcohol-95% alcohol-90% alcohol-80% alcohol-70% alcohol, Similar to the principle of dissolving.Generally in each reagent put 10 min, the weather can be a few minutes less heat, on the contrary, the colder weather, it is necessary to extend the dewaxing time, usually 12-15 min.
6, antigen repair
After dewaxing, rinse in clean water for a period of time, add 3% H2O2 for 10 min to remove endogenous catalase, then drained H2O2, wash twice in clear water, add citric acid buffer, Microwave cooking 3 min (medium heat), usually just to boil, cooled to room temperature, and then digested again, cooled to room temperature, the purpose of cooking is to make the antigen site exposed.
7, serum closed
After cooling to room temperature, the citrate buffer was drained, washed twice with water, and the slide was placed in PBS for 5 min, washed 2 times, the PBS solution around the tissue was dried, and immediay added serum to make some non-specific The site was closed and then placed in a 37 oC incubator for half an hour. Serum was diluted 10-fold (900 ul PBS: 100 ul serum blocking solution).