肺炎克雷伯菌/铜绿假单胞菌联合检测试剂盒

肺炎克雷伯菌/铜绿假单胞菌联合检测试剂盒（PCR方法）

广州健仑生物科技有限公司

One tube multiplex for detection AND quantification of Klebsiella pneumoniae, Pseudomonas aeruginosa and internal control.
单管多重检测和定量肺炎克雷伯菌，铜绿假单胞菌和内控。

肺炎克雷伯菌/铜绿假单胞菌联合检测试剂盒（PCR方法）

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（PCR方法）

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【公司名称】 广州健仑生物科技有限公司
【市场部】    杨永汉

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【腾讯  】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室

7、细胞核着色
不适当的组织处理可以出现细胞核着色，如组织在二甲苯里浸泡时间太长（如从星期五浸泡到下星期一）、缓冲液中浸泡时间太长、组织变干、微波修复液的PH值和修复时间不当或修复过程中修复液留下得太少，未没过组织等。解决办法是严格按照操作常规进行工作。
1、洗载玻片
将载玻片置于重铬酸钾和浓H2SO4混合液中，目的是为了是载玻片上的硅胶等除去，同时使一些肉眼看不见的凹凸不平的表面变平整，便于组织吸附，然后置于清水中清洗，除去残余的重铬酸钾和浓H2SO4（大约冲一个小时左右），再将载玻片浸泡于酒精之中， 然后放到架子上，置于37 oC温箱中，将多聚赖氨酸涂布于玻片的表面，由于Lys带正电，而大多数的组织带负电荷，从而产生吸附作用。
2、包埋组织
先在铁模具中加入一些液态石蜡，先稍微冷却，然后再将待包埋的组织置于石蜡之中，并排列整齐，再将塑料模具盒盖上，zui后加入少许液体石蜡，进行冷冻，使石蜡变成固态。
3、切片
将包埋好的组织从模具上取下来，并置于石蜡切片机上，切片机通过调节上下左右来来使组织和切割方向*，然后调节切片的厚度，一般为5 um，如果比较难切，则可以适当调整厚度，用毛笔将切割的载玻片向外拉，并用小镊子将包含有完整组织的载玻片置于40 oC温水中。

7, nuclear staining
Improper tissue handling can cause staining of the nucleus, such as tissue soaking in xylene for too long (eg soaking from Friday until next Monday), soaking in buffer for too long, tissue drying, and the pH of the microwave repair fluid and Repair time improper repair process or repair liquid left too little, have not been organized and so on. The solution is to strictly follow the routine work.
1, wash slides
Slides were placed in a mixture of potassium dichromate and concentrated H2SO4, the purpose is to remove the silica gel on the slide, etc., while some of the naked eye can see the uneven surface smooth, easy tissue adsorption, and then placed Clean water, remove the residual potassium dichromate and concentrated H2SO4 (about an hour or so), and then slides immersed in alcohol, and then placed on a shelf, placed in a 37 oC incubator, the poly Lysine is applied to the surface of glass slides. Since Lys is positively charged, most of the tissues are negatively charged, resulting in adsorption.
2, embedded tissue
First in the iron mold by adding some liquid paraffin, the first slightly cooled, then the tissue to be embedded in paraffin and arranged in neat rows, and then plastic mold cover, and finally add a small amount of liquid paraffin, frozen so that Paraffin becomes solid.
3, slice
The embedded tissue removed from the mold and placed on a paraffin slicer, slicer by adjusting the up and down to make the organization and cutting direction, and then adjust the thickness of the slice, usually 5 um, if more difficult to cut, The thickness can be adjusted appropriay, the cut slide is pulled outward with a brush and the slide containing the entire tissue is placed in 40 ° C warm water with a small forceps.