4种非典型肺炎联合检测试剂盒（PCR方法）

4种非典型肺炎联合检测试剂盒（PCR方法）

广州健仑生物科技有限公司

One tube multiplex for detection of Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella pneumophila/ Legionella longbeachae and internal control
单管多重检测肺炎支原体，肺炎衣原体，嗜肺军团杆菌，长滩军团菌和内控

4种非典型肺炎联合检测试剂盒（PCR方法）

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5、间质着色
着色部位主要在间质，间质着色的原因很多，如抗体与组织中的蛋白质因蛋白疏水基团相互作用形成非特异性的连接而着色，加一抗前的血清封闭这一步就是为了避免非特异型的结合。又如血清中的免疫球蛋白常常渗出到组织间质，很容易与抗体结合，造成间质着色，特别是lambda和kappa染色时。当甲状腺胶质外溢到组织间质时，做甲状腺球蛋白染色也会出现间质着色。抗体不纯或抗体被污染也可出现间质着色，我们曾遇到CD20抗体不纯，除了染上B细胞外还染上了间质。
6、细胞浆着色
胞浆着色是所有“杂音”染色中有欺骗性的着色，着色区局限在细胞内，间质无着色，看上去与真实的免疫反应着色几乎一样，很难区别。胞浆里含有较多的蛋白质，因此，很多非特异性的染色除了见于间质也可以出现在胞浆中。这种原因造成的着色，可以通过血清封闭解决。还有因内源酶造成的着色，如血红蛋白（红细胞）、肌红蛋白（肌细胞）、细胞色素（粒细胞、单核细胞）、过氧化氢酶（肝、肾），这些可用过氧化氢进行封闭。巨噬细胞吞噬各种抗原物质或Fc片断而出现胞浆着色，这种着色不易避免，但可以通过形态学辨认出巨噬细胞而引起重视。内源性生物素的着色有欺骗性，因为它广泛的存在于组织细胞中，我们研究结果显示：冰冻组织中存在内源性生物素，经福马林固定石蜡包埋后生物素被封闭，加热抗原修复后造成内源性生物素暴露，内源性生物素暴露的强度在不同的组织有所不同，从弱阳性（+）到强阳性（+++），内源性生物素在组织中的分布形式，既有散在分布也有弥漫分布，主要以颗粒状形式存在于胞浆中，内源性生物素广泛存在于上皮源性组织，特别是腺上皮组织，亦存在部分非上皮组织，内源性生物素不仅存在人体组织也存在大鼠组织，内源性生物素暴露的强弱与修复液有关，其强度增加依次为：柠檬酸、EDTA、EGTA，热抗原修复暴露的内源性生物素可被鸡蛋清封闭，非生物素检测系统Polymer两步法（EliVision、EnVision）可避免生物素干扰。

5, interstitial coloring
Coloring sites mainly in the interstitial interstitial coloration for many reasons, such as antibodies and proteins in the tissue due to protein hydrophobic groups interact to form a non-specific connection and coloring, plus a pre-anti-serum before the step of this step is to avoid non-specific Type of combination. Another example is the serum immunoglobulin often exudes to the interstitial tissue, it is easy to combine with antibodies, causing interstitial staining, especially lambda and kappa staining. When the thyroid gland spill to the interstitial tissue, do thyroid globulin stain will appear interstitial staining. Antibody impure or antibody contamination can also occur interstitial staining, we have encountered CD20 antibody impure, in addition to B cells infected with the interstitial.
6, cytoplasm coloring
Cytoplasmic staining is the most deceptive coloring of any "murmur" staining. The pigmentation zone is confined within the cell with no staining in the stroma. It appears to be almost the same as the actual immune response, which is difficult to distinguish. Cytoplasm contains more protein, therefore, a lot of non-specific staining in addition to the stroma can also appear in the cytoplasm. Coloring caused by this reason can be solved by serum blocking. There are also endogenous enzymes caused by the coloring, such as hemoglobin (red blood cells), myoglobin (myocytes), cytochromes (granulocytes, monocytes), catalases (liver, kidney), these available hydrogen peroxide Closed. Macrophage phagocytosis of various antigenic material or Fc fragment cytoplasmic staining, this coloring is not easy to avoid, but macrophages can be identified by morphological attention. Pigmentation of endogenous biotin is the most deceptive because of its widespread presence in tissue cells. Our results show that endogenous biotin is present in the frozen tissue and that biotin is blocked after the paraffin-embedded paraffin-embedded , Heat antigen repair caused endogenous biotin exposure, endogenous biotin exposure intensity varies in different tissues, from weak positive (+) to strongly positive (+++), endogenous biotin in Tissue distribution, both scattered and diffuse distribution, mainly in granular form exists in the cytoplasm, endogenous biotin widespread in epithelial tissue, especially glandular epithelial tissue, there are also some non-epithelial tissue Endogenous biotin not only existed in human tissues but also existed in rat tissues. The strength of endogenous biotin exposure was related to repair fluid, and the intensities of endogenous biotin were as follows: citric acid, EDTA, EGTA, endogenous Biotin can be blocked with egg white, non-biotin detection system Polymer two-step method (EliVision, EnVision) to avoid biotin interference.