8种细菌性肺炎多重检测试剂盒（PCR方法）

8种细菌性肺炎多重检测试剂盒（PCR方法）

广州健仑生物科技有限公司

Two tube multiplex for detection of Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, Staphylococcus aureus, Mycoplasma pneumoniae, Chlamydia pneumoniae, Legionella pneumophila/ Legionella longbeachae and internal control.
双管多重检测肺炎链球菌，流感嗜血杆菌，粘膜炎莫拉氏菌，金黄色葡萄球菌，肺炎支原体，肺炎衣原体，嗜肺军团杆菌，长滩军团菌和内部对照。

8种细菌性肺炎多重检测试剂盒（PCR方法）

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3、“阴阳脸”着色
指组织一半着色一半无着色，形成交界清晰或不甚清晰的两种染色结果。其成因是试剂仅覆盖了部分组织而不是全部。如加试剂后未让试剂流散开而集中在部分组织上。通常应该在加完试剂后，仔细看一遍，是否有的组织未被试剂*覆盖，如有这种情况，建议用牙签而不是用吸头或试剂瓶口将试剂引流开使之将组织全部覆盖。另外，染片盒不平，切片倾斜，虽然开始试剂已全部覆盖了组织，但后来试剂流向一边，部分组织未被试剂覆盖。对于这种问题，只要留心或想到了很容易发现，也很容易解决。有时，用DAKO（或PAP）笔在组织周围画圈时，划线太靠近或画到了组织上，由于笔油的力学原理，试剂不能达到靠近划线附近的组织。还有气泡也可引起阴阳分明的着色，只是不着色区域是圆形，由于气泡中含气，试剂被推到周围，因此，气泡中心的组织不着色。解决办法是滴加试剂时手法要轻，有气泡时用牙签捅破。
4、灶片状着色
切片中着色区东一块西一块，呈灶片状分布，出现这种问题的原因有：（1）裱片时水未排尽，在局部形成气泡使组织突起，染色时试剂渗入后不易洗尽，显色过深所致。解决办法是，漂片盒里的气泡应去尽，晾片热台不能平放，应有45度左右的斜度，利于水流走和蒸发。（2）坏死组织灶，组织坏死后细胞破坏、酶的释放、蛋白游离、分解，复杂的肽链残段（如Fc段）可能与一抗或/和二抗结合导致zui终着色。解决办法是在选择染色切片时应避免选择坏死组织较多的切片。（3）制作APES胶片时，胶的浓度太高，干燥后在玻片上留下白色小点，显色时白色小点着色。解决办法是按照标准的制备方法进行，即5%盐酸酒精（5 ml盐酸+95%酒精95 ml）浸泡玻片4小时、热水冲洗玻片1小时、蒸馏水洗玻片1分钟、丙酮浸泡玻片5秒钟后空气干燥（室温）、2% APES（2 ml APES+98 ml丙酮）浸泡玻片5分钟、玻片过一下丙酮（1-2秒钟）、玻片过一下蒸馏水（1-2秒钟）、37 度过夜干燥、室温储存备用。如果制片过程中，因丙酮逐渐挥发而胶变浓时可适当加入一些丙酮。

3, "Yin and Yang Face" coloring
Refers to the organization half of the color half without coloring, forming a clear or unclear junction of the two staining results. The reason is that the reagent covers only part of the tissue but not all. Such as adding reagents did not let the reagent flow and focus on part of the organization. Usually after adding the reagent, carefully read it again. Is there any tissue that is not fully covered by the reagent? In such cases, it is advisable to use a toothpick instead of using a tip or a reagent bottle to drain the reagent so that the entire tissue is covered . In addition, the cartridges were uneven and the slides were sloped. Although initially the reagent had compley covered the tissue, the reagents then flowed to one side, and some of the tissue was uncoated by the reagents. For such problems, as long as the care or thought of it is easy to find, but also very easy to solve. Sometimes, with a DAKO (or PAP) pen around the tissue, the scribe line is too close to or drawn onto the tissue, and because of the mechanics of the pen oil, the reagent can not reach the tissue near the scribe line. There are also bubbles can also cause a clear shade of yin and yang, but the non-colored area is round, because the bubbles in the gas, the reagent is pushed around, therefore, the center of the bubble tissue is not colored. The solution is to drop the reagent when the method is lighter, with a toothpick when pierced with bubbles.
4, stovepipe coloring
Eastern section of the coloring area of ​​a piece, was distributed in the form of a heart-shaped plate, the reasons for such problems are as follows: (1) when the water is not drained sheeting, the formation of local bubbles in the tissue protrusion, staining difficult to wash after reagent infiltration , Color due to too deep. The solution is, the bubble in the box should go to do, drying tablets can not be put flat heat, should be about 45 degrees of inclination, which will help the water flow and evaporation. (2) necrotic tissue, cell damage after tissue necrosis, enzyme release, protein free, decomposition, complex peptide chain fragments (such as Fc fragment) may be combined with the primary antibody or / and secondary antibody resulting in the final color. The solution is to avoid the need to select more sections of necrotic tissue when choosing a stained section. (3) When making APES film, the concentration of glue is too high, leaving small white spots on the glass slides after drying, and coloring of the white dots when coloring. The solution is according to the standard preparation method, namely 5% hydrochloric acid alcohol (5 ml hydrochloric acid + 95% alcohol 95 ml) for 4 hours soaking the glass slides, hot water slurries for 1 hour, distilled water, slides for 1 minute, acetone immersion glass After 5 seconds, the sample is air-dried (room temperature), 2% APES (2 ml APES + 98 ml acetone) for 5 minutes, slides in the glass for 1-2 seconds, slides in distilled water (1- 2 seconds), 37 degrees overnight dry, room temperature reserve. If the production process, due to the gradual evaporation of acetone and glue thickening may be appropriate to add some acetone.