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乙型流感IFA IgG荧光试剂盒
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除了这项研究之外，去年来自加州大学旧金山分校的研究人员提出了一个相似的名称：CRISPRi，他们发现当缺失核酸内切酶活性的Cas9与一种导向RNA共表达时候，会产生一种DNA识别复合物，这种复合物能特异性干扰转录延伸，RNA聚合酶结合，或转录因子结合。
由此研究人员研发出了这种CRISPRi系统，这一系统能有效抑制大肠杆菌中靶向基因的表达，并且不会出现脱靶效应。而且利用CRISPRi，还可以同时抑制多个靶基因，这种作用也是可逆。研究人员还证明，该系统也适用于哺乳动物细胞中的基因表达抑制。
加州大学圣迭戈分校(University of California, San Diego)医学院研究人员针对自闭症研究出一套比较新锐的理论，并对其进行了实际测试。研究结果显示，自闭症实际上是由异常的细胞通讯导致的，使用问世已逾百年的治疗嗜睡症的药物，可以恢复患有自闭症的老鼠体内的细胞活动，消除老鼠的神经失调症状。参与实验的老鼠年龄相当于人类30岁左右。相关研究成果在2014年6月17日的在线刊物《平移精神病学(Translational Psychiatry)》中发表。
医学、儿科与病理学教授、罗伯特·纳维奥(Robert K. Naviaux)博士为该研究的主要负责人，他表示：“这一发现与此前认为的自闭症是由内部原因导致的理论十分契合。在已知的与自闭症有关的致病原因中，有20%的遗传因素，但大部分病因并非如此。将基因与环境分离开思考并不合理。实际上，基因与环境会互相作用，作用的结果就是新陈代谢。”
纳维奥还提到：“自闭症的普遍症状是代谢紊乱。细胞有代谢物环(halo of metabolites)和核苷酸环绕，所谓的代谢物环是由参与新陈代谢的微小分子组成的。”
In addition to the study, researchers from the University of California, San Francisco, last year proposed a similar name, CRISPRi, who found that a DNA lacking endonuclease co-expressed with a targeting RNA produced a DNA Recognition complexes that specifically interfere with transcriptional elongation, RNA polymerase binding, or transcription factor binding.
As a result, the researchers developed the CRISPRi system, which effectively inhibits the expression of the targeted genes in E. coli and does not show off-target effects. And using CRISPRi, you can also inhibit multiple target genes, this effect is also reversible. The researchers also demonstrated that the system is also suitable for gene expression inhibition in mammalian cells.
Researchers at the University of California, San Diego Medical School developed a set of cutting-edge theories of autism and conducted practical tests. The results show that autism is actually caused by abnormal cell communication, the use of more than a century has come out of the treatment of narcolepsy drugs, can restore autism in mice with cell activity in vivo, to eliminate symptoms of neurological disorders in mice . The age of the mice involved in the experiment is about 30 years old. Relevant research results are published in the June 17, 2014 online translation "Translational Psychiatry."
Robert K. Naviaux, MD, Ph.D., professor of medicine, pediatrics and pathology, is the lead investigator for the study. "The finding and the previously thought autism is due to internal causes Fit in. Among the known causes of autism-related causes of disease, there are 20% of genetic factors, but most of the causes are not the same genetically isolated from the environment is not reasonable thinking.In fact, the genes and the environment will be mutually Effect, the result of the role of metabolism. "
Navio also mentioned: "The most common symptom of autism is metabolic disorder, where cells have halo of metabolites and nucleotides that are made up of tiny molecules that are involved in metabolism."
