腌菜添加违禁品检测试纸

腌菜添加违禁品检测试纸

广州健仑生物科技有限公司

主营品牌：美国US、美国Alfa、美国NovaBios、美国Cortez、国产创仑等等。

主要用途：筛查违禁品滥用残留、麻醉类药物残留、兴奋类药物残留等等。

独立包装：BZO-BAR-COC--THC -MET--OPI-OXY-MDMA-PCP- AMP-XTC-MTD 或联检

腌菜添加违禁品检测试纸

The One Step Drug Screen Test Card is an immunoassay based on the principle of competitive binding. Drugs which may be present in the urine specimen compete against their respective drug conjugate for binding sites on their specific antibody.

During testing, a urine specimen migrates upward by capillary action. A drug, if present in the urine specimen below its cut-off concentration, will not saturate the binding sites of its specific antibody. The antibody will then react with the drug-protein conjugate and a visible colored line will show up in the test line region of the specific drug strip. The presence of drug above the cut-off concentration will saturate all the binding sites of the antibody.  Therefore, the colored line will not form in the test line region.

A drug-positive urine specimen will not generate a colored line in the specific test line region of the strip because of drug competition, while a drug-negative urine specimen will generate a line in the test line region because of the absence of drug competition.

To serve as a procedural control, a colored line will always appear at the control line region, indicating that proper volume of specimen has been added and membrane wicking has occurred.

The test contains a membrane strip coated with drug-protein conjugates (purified bovine albumin) on the test line, a goat polyclonal antibody against gold-protein conjugate at the control line, and a dye pad which contains colloidal gold particles coated with mouse monoclonal antibody specific to Cocaine and Marijuana.

• For healthcare professionals including professionals at point of care sites.
• For in vitro diagnostic use only. Do not use after the expiration date.
• The test panel should remain in the sealed pouch until use.
• All specimens should be considered potentially hazardous and handled in the same manner as an infectious agent.
• The used test card should be discarded according to federal, state and local regulations.
• OPI一步试验条是一种横向流动色谱免疫分析法，用于在2000 ng / mL的截留浓度下检测尿液中的OPI制剂。
  该测定仅提供初步的分析测试结果。 必须使用更具体的替代化学方法才能获得确认的分析结果。 气相色谱/质谱（GC / MS）是优选的确认方法。 临床考虑和专业判断应适用于任何滥用药物的滥用测试结果，特别是当使用初步的肯定结果时。

【检验方法】

在进行检测前必须先完整阅读使用说明书，使用前将本品和尿样恢复至室温（20℃～30℃）。

• 撕开铝箔袋，取出试剂盒，应在1小时内尽快使用。
• 将试剂盒置于干净平坦的台面上，用塑料吸管垂直滴加3滴无空气泡的尿样（约100µL）于加样孔（S）中。
• 等待紫红色条带的出现，3～5分钟时直接观察结果，10分钟后判定无效。

【参考值（参考范围）】

本品zui低检出量指标参照美国药物滥用和精神健康服务管理局(SAMHSA)确定的阳性检测临界浓度的标准进行制定。能检测出尼古丁含量不低于300ng/mL的样本。

【检验结果的解释】

阳性（+）：仅在控制区（C）出现一条紫红色条带，在检测区（T）无紫红色条带出现。阳性结果表明尿液中的尼古丁浓度在阈值（300ng/mL）以上。

阴性（-）：出现两条紫红色条带。一条位于检测区（T），另一条位于控制区（C）。阴性结果表明尿液中的尼古丁浓度在阈值（300ng/mL）以下。

无效：控制区（C）未出现紫红色条带。表明操作不当或试剂盒已失效。在此情况下，应再次仔细阅读说明书，并用新的试剂盒重新测试。如果问题仍然存在，应立即停止使用此批号产品，并与当地供应商。

注意：检测区（T）紫红色条带可呈现颜色深浅的现象。但是，在规定的观察时间内，不论该色带颜色深浅，即使只有非常弱的色带也应判定为阴性结果。

美国NOVABIOS多联检测杯简介：

美国NOVABIOS单卡产品简介：

传统基于PCR的克隆方法或者通过构建基因组文库筛选等方法存在着克隆片段长度的限制、易产生突变、步骤复杂、效率低下等缺点，并不适合大片段基因组的克隆。因此开发一种快速简单的方法来获得大尺度的基因簇片段至关重要，这也是目前许多实验室的课题之一。
近日，中科院微生物所娄春波课题组与清华大学、特拉维夫大学合作开发了利用CRISPR-Cas9系统一步靶向克隆上百kb基因簇片段的方法。CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) /Cas (CRISPR-associated) 是近年出现的一种由RNA介导Cas核酸酶对靶向基因进行特定DNA修饰的技术。它是细菌和古细菌为应对细菌毒和质粒的不断攻击而演化来的获得性免疫防御机制。
该系统的工作原理是crRNA (CRISPR-derived RNA) 通过碱基配对与 tracrRNA (trans-activating RNA) 结合形成 tracrRNA/crRNA 复合物，此复合物引导核酸酶 Cas9 蛋白在与 crRNA 配对的序列靶位点处剪切双链 DNA，从而实现对基因组DNA序列进行编辑；而通过人工设计这两种 RNA，可以改造形成具有引导作用的gRNA (guide RNA)，足以引导 Cas9 对 DNA 的定点切割。Cas9 能在任何 dsDNA 序列处带来任何融合蛋白及RNA，这为生物体的研究和改造带来巨大潜力。
基于CRISPR-Cas9系统的原理，研究人员利用RNA介导的CRISPR-Cas9核酸内切酶精确的切割所需克隆的任意基因簇片段两侧，并通过Gibson组装方法将切割下来的大片段与目标载体进行连接，达到一步克隆的目的。利用该方法成功克隆了不同尺度（50-150 kb）的大肠杆菌基因组片段，并在其他细菌（枯草芽孢杆菌、链霉菌）中成功克隆了芽孢、金霉素、杰多霉素等生物合成基因簇。
该技术的成功，将大大促进功能基因组学的研究，以及其它需要进行大片段克隆的研究。目前，该技术已于2015年9月1日在Nature Communications 杂志在线发表，文章*作者是清华大学博士生姜文君，微生物所赵学金博士做出了前期的重要贡献，并PCR（聚合酶链式反应）是分子生物学实验室细菌见技术，也是DNA测序的必须步骤。PCR技术自出现以来，就被广泛用于生物学的各个研究领域，对生物学的发展起到了极大的推动作用。然而，相比于其他分子技术，PCR技术出现以后发展缓慢，至今没有大的改进与创新，是细菌通量测序与规模化检测的一个限制因素。

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【公司名称】 广州健仑生物科技有限公司

【 市场部 】       杨永汉
【】 
【腾讯  】
【公司地址】 广州市清华科技园健新基地番禺石楼镇健启路63号二期2幢101-103室

Traditional PCR-based cloning methods or the construction of genomic library screening methods have the limitations of cloning fragment length, easy to produce mutations, complex steps, low efficiency and other shortcomings, is not suitable for large fragment cloning of the genome. Therefore, it is of crucial importance to develop a fast and simple method to obtain large-scale gene cluster fragments, which is also one of the topics in many laboratories at present.
Recently, Lou Chunbo, a member of the Chinese Academy of Sciences Institute of Microbiology, collaborated with Tsinghua University and Aviv University to develop a method for the targeted cloning of hundreds of kb gene cluster fragments using the CRISPR-Cas9 system. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) / Cas (CRISPR-associated) is a technology that has been used to perform specific DNA modification of target genes by RNA-mediated Cas nuclease in recent years. It is an acquired immune defense mechanism that bacteria and archaebacteria evolve in response to the constant attack of bacterial viruses and plasmids.
The system works by crRNA (CRISPR-derived RNA) binding to tracrRNA (trans-activating RNA) by base pairing to form a tracrRNA / crRNA complex that directs the expression of the nuclease Cas9 protein at the sequence target site paired with the crRNA Double-stranded DNA cut in order to achieve the editing of the genomic DNA sequence; and by artificial design of these two RNA, you can transform the formation of a guide gRNA (guide RNA), enough to guide Cas9 site-directed DNA cleavage. Cas9 can bring in any fusion protein and RNA at any dsDNA sequence, which has great potential for the research and modification of organisms.
Based on the principle of the CRISPR-Cas9 system, the researchers used RNA-mediated CRISPR-Cas9 endonucleases to precisely cut both sides of any gene cluster fragment of the desired clone and ligated the cut large fragment to a target vector To connect, to achieve the purpose of one-step cloning. Using this method, we successfully cloned Escherichia coli genome fragments of different sizes (50-150 kb) and successfully cloned the biosynthetic genes of spore, chlortetracycline and gentamycin in other bacteria (Bacillus subtilis and Streptomyces) cluster.
The success of this technology will greatly facilitate functional genomics research and other studies that require large fragment cloning. At present, this technology was published online in Nature Communications magazine on September 1, 2015. The lead author of this article is Jiang Wenjun, a doctoral student at Tsinghua University, and Dr. Zhao Xuejin from the Institute of Microbiology made an important contribution in the previous period. PCR (Polymerase Chain Reaction) is a molecular biology laboratory see the technology of bacteria, but also a necessary step in DNA sequencing. Since its emergence, PCR technology has been widely used in various fields of biology and has greatly promoted the development of biology. However, compared with other molecular technologies, PCR technology has been slow to develop since the advent of PCR technology, and so far no major improvements and innovations have been identified as a limiting factor for bacterial flux sequencing and mass detection.