NOVABIOS乙脑IGM抗体检测试剂盒
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NOVABIOS乙脑IGM抗体检测试剂盒用于暴露于日本脑炎病毒（JEV）是用于检测人血清中的IgE抗体对JEV衍生的重组抗原（JERA）（1-4）的ELISA测定系统。这个测试是为了帮助诊断人类暴露于日本脑炎病毒（JEV）。它不是为了筛选血液或血液成分，仅用于专业的体外诊断用途。该试剂盒尚未针对疫苗诱导的血清学转换研究进行优化。
- 产品描述
NOVABIOS乙脑IGM抗体检测试剂盒
JE DetectTM IgM ANTIBODY
CAPTURE ELISA (MAC-ELISA)
INTENDED USE
The JE Detect MAC-ELISA test for exposure to Japanese Encephalitis Virus (JEV) is an ELISA assay system for the detection of IgM antibodies in human serum to JEV derived recombinant antigen (JERA) (1-4). This test is to aid in the diagnosis of human exposure to the Japanese Encephalitis Virus (JEV). It is not intended to screen blood or blood components, and is for professional in vitro diagnostic use only.
SUMMARY AND EXPLANATION OF THE TEST
Exposure to JEV causes a disease with a number of symptoms including encephalitis (5-8). The JE Detect MAC-ELISA employs a recombinant antigen called JERA, which can be used as a rapid serological marker for JEV infection. The JERA protein is a recombinant antigen, which consists of a stretch of peptides from different parts of the JEV antigens.
NOVABIOS乙脑IGM抗体检测试剂盒
PRINCIPLE OF THE TEST
The JE Detect MAC-ELISA consists of one enzymatically amplified "two-step" sandwich-type immunoassay.
In this assay, JE Detect Negative Control (Represents non-reactive serum), JE Detect IgM Positive Control (Represents reactive serum), and unknown serum samples are incubated in microtitration wells which have been coated with anti-human IgM antibodies, followed by incubation with both JEV derived recombinant JERA and Normal Cell Antigen (NCA) separay. The serum samples may be diluted with Sample Dilution Buffer for JE Detect IgM. After incubation and washing, the wells are treated with a JERA-specific antibody labeled with the enzyme horseradish peroxidase (HRP). After a second incubation and washing step, the wells are incubated with the tetramethylbenzidine (TMB) substrate.
An acidic stopping solution is then added and the degree of enzymatic turnover of the substrate is determined by absorbance measurement at 450 nanometers. Above a certain threshold, the ratio of the absorbencies of the JERA and the control wells accuray determines whether antibodies to JEV are present.
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【公司名称】 广州健仑生物科技有限公司
【】 杨
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【腾讯 】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-103室
